Rhodococcus opacus B4

Gram-positiveCocciNon-motileAerobe

Kingdom

Bacillati

Phylum

Actinomycetota

Class

Actinomycetes

Order

Mycobacteriales

Family

Nocardiaceae

Genus

Rhodococcus

Description

Rhodococci are aerobic, Gram positive actinomycetes of high G+C content, capable of morphological differentiation in response to their environment (e.g., cocci or filaments). These widely occurring organisms are of considerable environmental and biotechnological importance due to their broad metabolic diversity and array of unique enzymatic capabilities. These are of interest to the pharmaceutical, environmental, chemical and energy sectors. Specific applications include the desulphurization of fossil fuels and the industrial production of acrylamide. Rhodococci are well suited for bioremediation due to their capacity for long term survival in soil, their exceptional ability to degrade hydrophobic pollutants even in the presence of more readily assimilable carbon sources, and their ability to accumulate high levels of heavy metals.Strain B4 was isolated from a soil sample taken from the roadside in Hiroshima, Japan. It is a benzene-tolerant, Gram-postitive, non-motile, strictly aerobic rod, able to use many organic compound as its sole source of carbon and energy (adapted from PubMed 16233805). (HAMAP: RHOOB)

Taxonomy

KingdomBacillati
PhylumActinomycetota
ClassActinomycetes
OrderMycobacteriales
FamilyNocardiaceae
GenusRhodococcus
SpeciesRhodococcus opacus
StrainB4

Profile

Physiology
Gram staining propertiesPositive
ShapeCocci
MobilityNo
Flagellar presenceYes
Number of membranes1
Image of Rhodococcus opacus B4
AI-generated image based on bacteria physiology
Ecology, Host, and Life Cycle
Oxygen requirementsAerobe
Optimal temperatureNot Available
Temperature rangeMesophilic
HabitatNot Available
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementFilaments
SporulationNonsporulating
Energy sourceNot Available
PathogenicityNo

Gene Summary

Adenine Count

756 bp

Thymine Count

793 bp

Guanine Count

1414 bp

Cytosine Count

1404 bp

Genome Length

4367 bp

Protein-coding Genes

6 genes

Non-Coding Genes

0 genes

# of Chromosomes/Plasmids

6

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
phou domain-containing proteinROP_RS44995Not AvailablePositive640 - 8467167.4
clp protease n-terminal domain-containing proteinROP_RS00005Not AvailablePositive899 - 130314671.6
type ii toxin-antitoxin system hicb family antitoxinROP_RS00010Not AvailableNegative1496 - 186113004.2
nucleotidyltransferase domain-containing proteinROP_RS00020Not AvailablePositive2534 - 316022943.2
hypothetical proteinROP_RS00025Not AvailablePositive3160 - 356714561.2
hypothetical proteinROP_RS44750Not AvailablePositive3981 - 41064488.7
mfs transporterROP_RS00030Not AvailableNegative5167 - 643543695.3
fad-dependent oxidoreductaseROP_RS00035Not AvailableNegative6440 - 779847449.0
metalloregulator arsr/smtb family transcription factorROP_RS00040Not AvailablePositive7901 - 877031248.1
merr family transcriptional regulatorROP_RS00045Not AvailablePositive8841 - 961427778.2

Displaying genes 1 – 10 of 8246 in total

Metabolites

1 record
Metabolite IDMetabolite nameStructureCAS number
BASm0014029(S)-3-Hydroxyisobutyric acidC19H35N5O6SeChemical structure of (S)-3-Hydroxyisobutyric acid26543-05-5
Average508.489Da
Monoisotopic509.175256Da

Displaying 1–1 of 1 metabolites

Health Effects

No health effects information available for this bacterium.