Cronobacter sakazakii strain ATCC 29544

Gram-negativeRodNon-motileAnaerobe

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Gammaproteobacteria

Order

Enterobacterales

Family

Enterobacteriaceae

Genus

Cronobacter

Description

Cronobacter sakazakii strain ATCC 29544 is a Gram-negative, rod-shaped bacterium that is classified as an anaerobe, indicating its capacity to thrive in environments devoid of oxygen. This strain is characterized by its non-motility and presence of flagella. It has been identified as a free-living organism, typically found in a host-associated habitat, and is mesophilic with an optimal growth temperature of 37°C. The strain has a complex genomic structure with four replicons, and it has been documented to inhabit various hosts, including Homo sapiens, Viridiplantae, Amphibia, Gastrotheca, and Cucumis sativus. Notably, this strain is recognized for its pathogenic potential in humans, linked to serious health issues such as meningitis, sepsis, septicemia, and necrotizing enterocolitis (NEC). It can also cause mild diseases and has been associated with brain abscesses or lesions. The ecological role of Cronobacter sakazakii strain ATCC 29544 is significant, as its ability to inhabit a variety of hosts suggests a versatile adaptability to different environmental conditions. This adaptability could play a critical role in its transmission dynamics and pathogenicity, particularly in vulnerable populations such as infants and immunocompromised individuals. Understanding the biology and ecology of this strain helps elucidate its impact on health and disease.

Taxonomy

KingdomPseudomonadati
PhylumPseudomonadota
ClassGammaproteobacteria
OrderEnterobacterales
FamilyEnterobacteriaceae
GenusCronobacter
SpeciesCronobacter sakazakii
Strainstrain ATCC 29544

Profile

Physiology
Gram staining propertiesNegative
ShapeRod
MobilityNo
Flagellar presenceYes
Number of membranesNot Available
Image of Cronobacter sakazakii strain ATCC 29544
Image source: Wikipedia/Wikimedia
Ecology, Host, and Life Cycle
Oxygen requirementsAnaerobe
Optimal temperature37
Temperature rangeMesophilic
HabitatHostAssociated
Biotic relationshipFree living
Host(s)Homo sapiens, Viridiplantae, Amphibia
Cell arrangementPairs - Singles
SporulationNonsporulating
Energy sourceNot Available
PathogenicityHuman

Genome Summary

Cronobacter sakazakii strain ATCC 29544 chromosome, complete

Gene Summary

Adenine Count

975935 bp

Thymine Count

976836 bp

Guanine Count

1278326 bp

Cytosine Count

1280168 bp

Genome Length

4511265 bp

Protein-coding Genes

4008 genes

Non-Coding Genes

406 genes

# of Chromosomes/Plasmids

4

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
carboxymuconolactone decarboxylase family proteinCSK29544_RS14245P76035Negative2996546 - 299770041600.7
enoyl-acp reductase fabiCSK29544_RS14250P16657Negative2997890 - 299867827971.7
putrescine export abc transporter atp-binding protein sapfCSK29544_RS14255P0AAH9Negative2998799 - 299960530481.4
putrescine export abc transporter atp-binding protein sapdCSK29544_RS14260P36636Negative2999607 - 300059937698.8
putrescine export abc transporter permease sapcCSK29544_RS14265P0AGH6Negative3000599 - 300148931558.1
putrescine export abc transporter permease sapbCSK29544_RS14270P0A2J4Negative3001476 - 300244135992.3
abc transporter substrate-binding protein sapaCSK29544_RS14275P36634Negative3002438 - 300407861154.4
phage shock protein operon transcriptional activatorCSK29544_RS14280P37344Negative3004176 - 300517737882.7
phage shock protein pspaCSK29544_RS14285P0AFM7Positive3005330 - 300600125405.1
envelope stress response membrane protein pspbCSK29544_RS14290P0AFN1Positive3006067 - 30062918797.58

Displaying genes 3091 – 3100 of 4541 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolites

263 records
Metabolite IDMetabolite nameStructureCAS number
BASm0000237(R)-4'-phosphopantothenateC9H18NO8PChemical structure of (R)-4'-phosphopantothenateNot available
Average299.2149Da
Monoisotopic299.0770031Da
BASm00002502,5-didehydro-D-gluconateC6H7O7Chemical structure of 2,5-didehydro-D-gluconate53736-12-2
Average191.1156Da
Monoisotopic191.019177578Da
BASm00002512-dehydropantoateC6H9O4Chemical structure of 2-dehydropantoateNot available
Average145.1333Da
Monoisotopic145.050083776Da
BASm0000274aldehydo-D-galacturonateC6H9O7Chemical structure of aldehydo-D-galacturonateNot available
Average193.132Da
Monoisotopic193.0353762Da
BASm0000377(S)-malateC4H4O5Chemical structure of (S)-malateNot available
Average132.0716Da
Monoisotopic132.005873238Da
BASm0000387(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6Chemical structure of (6R)-5,10-methylene-5,6,7,8-tetrahydrofolateNot available
Average455.432Da
Monoisotopic455.1564286Da
BASm0000401(S)-2-succinylamino-6-oxoheptanedioateC11H12NO8Chemical structure of (S)-2-succinylamino-6-oxoheptanedioateNot available
Average286.218Da
Monoisotopic286.0579371Da
BASm0000403(S)-acetoinC4H8O2Chemical structure of (S)-acetoinNot available
Average88.1051Da
Monoisotopic88.0524295Da
BASm00005275-oxopentanoateC5H7O3Chemical structure of 5-oxopentanoateNot available
Average115.109Da
Monoisotopic115.040067665Da
BASm0000642S-adenosyl-4-methylsulfanyl-2-oxobutanoateC15H19N5O6SChemical structure of S-adenosyl-4-methylsulfanyl-2-oxobutanoateNot available
Average397.406Da
Monoisotopic397.105604055Da

Displaying 1–10 of 263 metabolites

Health Effects

Health ConditionRelationReference
Mild diseasesCausesPMC5615607
Bacterial meningitisCausesPMC10370310
SepsisCausesPMC10370310
Necrotizing enterocolitisCausesPMC11267904
NecCausesPMC11267904
SepsisCausesPMC11267904
MeningitisCausesPMC11267904
Necrotizing enterocolitisCausesPMC1868726
BacteraemiaCausesPMC1868726
MeningitisCausesPMC1868726

Displaying health effects 1 – 10 of 19 in total