Pseudomonas putida strain MC4-5222

Gram-negativeRodMotileFacultative

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Gammaproteobacteria

Order

Pseudomonadales

Family

Pseudomonadaceae

Genus

Pseudomonas

Description

Pseudomonas putida strain MC4-5222 is a Gram-negative, rod-shaped bacterium that primarily inhabits soil and wastewater environments. This strain is heterotrophic, utilizing organic compounds as its energy source, and exhibits facultative anaerobic metabolism, allowing it to thrive in both aerobic and anaerobic conditions. P. putida MC4-5222 is motile, possessing flagella that facilitate its movement. The strain has a mesophilic temperature range, indicating optimal growth at moderate temperatures. It is characterized by a single replicon and possesses two membranes, typical of Gram-negative bacteria. P. putida MC4-5222 is free-living and has been associated with a diverse array of biological hosts, including various plants (e.g., Triticum aestivum, Solanum lycopersicum, Oryza sativa) and even animals (e.g., Homo sapiens, Struthio camelus). While this strain is not known to sporulate, it has been implicated in nosocomial infections, suggesting a potential pathogenic relationship with animal hosts. Its ability to thrive in various ecological niches and its interactions with a wide range of hosts emphasize the ecological versatility of Pseudomonas putida. This adaptability not only highlights its role in natural soil and wastewater ecosystems but also raises concerns regarding its presence in clinical settings, where it may contribute to health complications. The strain's varied interactions with both plant and animal life illustrate the complex dynamics of microbial life and its implications for health and disease.

Taxonomy

KingdomPseudomonadati
PhylumPseudomonadota
ClassGammaproteobacteria
OrderPseudomonadales
FamilyPseudomonadaceae
GenusPseudomonas
SpeciesPseudomonas putida
Strainstrain MC4-5222

Profile

Physiology
Gram staining propertiesNegative
ShapeRod
MobilityYes
Flagellar presenceYes
Number of membranes2
Image of Pseudomonas putida strain MC4-5222
Image source: Wikipedia/Wikimedia
Ecology, Host, and Life Cycle
Oxygen requirementsFacultative
Optimal temperatureNot Available
Temperature rangeMesophilic
HabitatSoil - Wastewater
Biotic relationshipFree living
Host(s)Homo sapiens, Viridiplantae, Triticum aestivum
Cell arrangementSingles
SporulationNonsporulating
Energy sourceHeterotroph
PathogenicityAnimal

Genome Summary

Pseudomonas putida strain MC4-5222


Gene Summary

Adenine Count

1383957 bp

Thymine Count

1378669 bp

Guanine Count

2049509 bp

Cytosine Count

2045961 bp

Genome Length

6858096 bp

Protein-coding Genes

5993 genes

Non-Coding Genes

131 genes

# of Chromosomes/Plasmids

1

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
Prophage repressorHA62_29675Not AvailableNegative331137 - 33178723856.3
Hypothetical proteinHA62_29680Not AvailablePositive331895 - 3321137966.36
Cii regulatory proteinHA62_29685Not AvailablePositive332116 - 33242411695.1
hypothetical proteinHA62_29690Not AvailableNegative332807 - 3330227649.05
hypothetical proteinHA62_29695Not AvailablePositive333040 - 33381929060.1
Holliday junction resolvaseHA62_29700Not AvailablePositive333812 - 33425515651.3
Hypothetical proteinHA62_29705Not AvailablePositive334391 - 33527232975.4
Hypothetical proteinHA62_29710Not AvailablePositive335422 - 33580813425.4
Hypothetical proteinHA62_29715Not AvailablePositive335801 - 33608510295.0
Glycoside hydrolase family 19HA62_29720Not AvailablePositive336085 - 33670522329.8

Displaying genes 1 – 10 of 6124 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolites

8 records
Metabolite IDMetabolite nameStructureCAS number
BASm0000377(S)-malateC4H4O5Chemical structure of (S)-malateNot available
Average132.0716Da
Monoisotopic132.005873238Da
BASm0001691hydrogenselenideHSeChemical structure of hydrogenselenideNot available
Average79.98Da
Monoisotopic80.924896Da
BASm0001865diphosphateHO7P2Chemical structure of diphosphateNot available
Average174.95Da
Monoisotopic174.9213971Da
BASm0001921(S)-3-methyl-2-oxopentanoateC6H9O3Chemical structure of (S)-3-methyl-2-oxopentanoate1460-34-0
Average129.1339Da
Monoisotopic129.0551692Da
BASm0002282(2R)-2,3-dihydroxy-3-methylbutanoateC5H9O4Chemical structure of (2R)-2,3-dihydroxy-3-methylbutanoateNot available
Average133.1226Da
Monoisotopic133.0500838Da
BASm0002307(2R,3R)-2,3-dihydroxy-3-methylpentanoateC6H11O4Chemical structure of (2R,3R)-2,3-dihydroxy-3-methylpentanoateNot available
Average147.1491Da
Monoisotopic147.06573384Da
BASm0003389NADP(+)C21H25N7O17P3Chemical structure of NADP(+)Not available
Average740.386Da
Monoisotopic740.053624107Da
BASm0003491(2S)-2-acetolactateC5H7O4Chemical structure of (2S)-2-acetolactateNot available
Average131.108Da
Monoisotopic131.0349823Da

Displaying 1–8 of 8 metabolites

Health Effects

Health ConditionRelationReference
Nosocomial infectionsCausesPMC11585281
Nosocomial infectionsCausesPMC13243026

Displaying health effects 1 – 2 of 2 in total