Cupriavidus metallidurans CH34

Gram-negativeRodMotileFacultative

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Betaproteobacteria

Order

Burkholderiales

Family

Burkholderiaceae

Genus

Cupriavidus

Description

Cupriavidus metallidurans CH34. This strain was first identified in the heavy metal-contaminated sludge of a settling tank in Belgium in the late 1970s. Metal resistance functions are predominantly encoded on two plasmids, pMOL28 and pMOL30, which produce metal exporters that pump metal ions out of the cell, thus protecting intracellular macromolecules from the toxic effects of high concentrations of metal. (NCBI BioProject: bp_list[1])

Taxonomy

Kingdom	Pseudomonadati
Phylum	Pseudomonadota
Class	Betaproteobacteria
Order	Burkholderiales
Family	Burkholderiaceae
Genus	Cupriavidus
Species	Cupriavidus metallidurans
Strain	CH34

Profile

Physiology
Gram staining properties	Negative
Shape	Rod
Mobility	Yes
Flagellar presence	Yes
Number of membranes	Not Available

AI-generated image based on bacteria physiology

Ecology, Host, and Life Cycle
Oxygen requirements	Facultative
Optimal temperature	30
Temperature range	Mesophilic
Habitat	Specialized
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Singles
Sporulation	Nonsporulating
Energy source	Not Available
Pathogenicity	Not Available

Genome Summary

Cupriavidus metallidurans CH34 plasmid pMOL28, complete sequence.

NC_007972.2 NC_007974.2 NC_007971.2 NC_007973.1

Gene Summary

Adenine Count

34395 bp

Thymine Count

33323 bp

Guanine Count

51333 bp

Cytosine Count

52408 bp

Genome Length

171459 bp

Protein-coding Genes

168 genes

Non-Coding Genes

0 genes

# of Chromosomes/Plasmids

Genes

Name	Locus Tag	UniProt ID	Strand Orientation	Gene Start/End	Protein Molecular Weight
is5-like element isrme6 family transposase	RMET_RS32090	Not Available	Positive	1425425 - 1426242	30905.4
uroporphyrinogen-iii c-methyltransferase	RMET_RS06610	Not Available	Negative	1426313 - 1426919	21623.6
2fe-2s iron-sulfur cluster-binding protein	RMET_RS06615	Not Available	Negative	1426985 - 1428007	37943.2
aromatic/alkene monooxygenase hydroxylase subunit beta	RMET_RS06620	Not Available	Negative	1428107 - 1429093	37915.6
mmob/dmpm family protein	RMET_RS06625	Not Available	Negative	1429148 - 1429462	11909.2
rieske 2fe-2s domain-containing protein	RMET_RS06630	Not Available	Negative	1429503 - 1429838	12319.7
toluene-4-monooxygenase system b family protein	RMET_RS06635	Not Available	Negative	1429894 - 1430160	9671.63
aromatic/alkene/methane monooxygenase hydroxylase/oxygenase subunit alpha	RMET_RS06640	Not Available	Negative	1430229 - 1431731	57778.2
2-hydroxymuconate tautomerase	RMET_RS06645	Not Available	Negative	1431791 - 1431982	7110.71
2-oxo-3-hexenedioate decarboxylase	RMET_RS06650	Not Available	Negative	1431994 - 1432782	27821.8

Displaying genes 4071 – 4080 of 6407 in total

Pathways

1335 pathways

2,3-Dihydroxybenzoate Biosynthesis

Biosynthesis of Siderophore Group Nonribosomal Peptides

Lysine Biosynthesis

Taurine Metabolism

TCA Cycle

Glycolysis and Pyruvate Dehydrogenase

Arginine Metabolism

Ornithine Metabolism

Show All 1335 Pathways

Metabolite ID	Metabolite name	CAS number	Average mass Monoisotopic mass
BASm0000250	2,5-didehydro-D-gluconateC6H7O7	`53736-12-2`	Average191.1156Da Monoisotopic191.019177578Da
BASm0000253	2-oxopent-4-enoateC5H5O3	Not available	Average113.093Da Monoisotopic113.024417601Da
BASm0000260	3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanateC24H37O5	Not available	Average405.556Da Monoisotopic405.264647871Da
BASm0000428	3-oxoadipateC6H6O5	Not available	Average158.11Da Monoisotopic158.022620453Da
BASm0000516	6-deoxyerythronolide BC21H38O6	Not available	Average386.5228Da Monoisotopic386.2668389Da
BASm0000553	biphenyl-2,3-diolC12H10O2	Not available	Average186.2066Da Monoisotopic186.0680796Da
BASm0000592	(S)-1-phenylethanolC8H10O	Not available	Average122.1644Da Monoisotopic122.0731649Da
BASm0000599	2,5-dihydroxypyridineC5H5NO2	Not available	Average111.1Da Monoisotopic111.0320284Da
BASm0000893	crotonobetaineC7H13NO2	`927-89-9`	Average143.1836Da Monoisotopic143.0946287Da
BASm0000989	GlycerolC3H8O3	`56-81-5`	Average92.0938Da Monoisotopic92.04734412Da

Displaying 1–10 of 1772 metabolites

Health Effects

No health effects information available for this bacterium.