Rhizobium leguminosarum bv. trifolii WSM1325

Gram-negativeRodMotileAerobe

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Alphaproteobacteria

Order

Hyphomicrobiales

Family

Rhizobiaceae

Genus

Rhizobium

Description

Rhizobium leguminosarum is a widely studied alpha-proteobacterial N2-fixing legume symbiont of legumes. It has three biovars - viciae, trifolii, phaseoli - that differ in their host specificity. The host-specificity genes are carried on large plasmids that also carry the genes for nitrogen fixation. All strains of R. leguminosarum have several large plasmids, but the number and sizes of plasmids varies among strains. This large number of plasmids distinguishes R. leguminosarum from the other rhizobia for which complete genome sequences have been determined.Strain WSM1325 was isolated in 1993 from a nodule recovered from the roots of an annual clover plant growing near Livadi beach on the Greek Cyclades island of Serifos. It is compatible with many perennial clovers of Mediterranean origin used in farming, such as T. pratense, and is therefore one of the most important clover inoculants. However, WSM1325 sometimes nodulates and never fixes nitrogen with American and African perennial clovers, such as those nodulated by WSM2304. It is important to understand this incompatibility (adapted from 10.4056/sigs.852027). (HAMAP: RHILS)

Taxonomy

KingdomPseudomonadati
PhylumPseudomonadota
ClassAlphaproteobacteria
OrderHyphomicrobiales
FamilyRhizobiaceae
GenusRhizobium
SpeciesRhizobium leguminosarum
StrainWSM1325

Profile

Physiology
Gram staining propertiesNegative
ShapeRod
MobilityYes
Flagellar presenceYes
Number of membranes2
Image of Rhizobium leguminosarum bv. trifolii WSM1325
Image source: Wikipedia/Wikimedia
Ecology, Host, and Life Cycle
Oxygen requirementsAerobe
Optimal temperatureNot Available
Temperature rangeMesophilic
HabitatSoil
Biotic relationshipSymbiotic
Host(s)Pisum sativum
Cell arrangementSingles
SporulationNonsporulating
Energy sourceChemoheterotroph
PathogenicityNo

Genome Summary

Rhizobium leguminosarum bv. trifolii WSM1325


Gene Summary

Adenine Count

131101 bp

Thymine Count

127879 bp

Guanine Count

197798 bp

Cytosine Count

204195 bp

Genome Length

660973 bp

Protein-coding Genes

655 genes

Non-Coding Genes

0 genes

# of Chromosomes/Plasmids

6

Genes

NameLocus TagUniProtStrandCoordinatesMolecular Weight
helix-turn-helix domain-containing proteinRLEG_RS26805Not Available+80 - 76325205.0
glutamine amidotransferase family proteinRLEG_RS26810O87390+852 - 176032080.1
gxgxg domain-containing proteinRLEG_RS26815O87391+1799 - 248523791.4
fmn-binding glutamate synthase family proteinRLEG_RS26820O87392+2500 - 382847434.8
type iii glutamate--ammonia ligaseRLEG_RS26825P31592+3912 - 521948227.3
formyltetrahydrofolate deformylaseRLEG_RS26830Q46339+5412 - 629632692.1
aminomethyltransferase family proteinRLEG_RS26835Q9K934+6446 - 757941795.2
dimethylamine monooxygenase subunit dmma family proteinRLEG_RS26840Not Available+7591 - 819621752.2
pdr/vanb family oxidoreductaseRLEG_RS26845D0C9N8+8193 - 915835416.8
duf3445 domain-containing proteinRLEG_RS26850Not Available+9190 - 1023040774.4

Displaying genes 1 – 10 of 5757 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolites

1760 records
Metabolite IDMetabolite nameStructureCAS number
BASm00002502,5-didehydro-D-gluconateC6H7O7Chemical structure of 2,5-didehydro-D-gluconate53736-12-2
Average191.1156Da
Monoisotopic191.019177578Da
BASm00002512-dehydropantoateC6H9O4Chemical structure of 2-dehydropantoateNot available
Average145.1333Da
Monoisotopic145.050083776Da
BASm00002532-oxopent-4-enoateC5H5O3Chemical structure of 2-oxopent-4-enoateNot available
Average113.093Da
Monoisotopic113.024417601Da
BASm00002543-hydroxy-2-methylpropanoateC4H7O3Chemical structure of 3-hydroxy-2-methylpropanoateNot available
Average103.098Da
Monoisotopic103.0400677Da
BASm00002603alpha,7alpha-dihydroxy-12-oxo-5beta-cholanateC24H37O5Chemical structure of 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanateNot available
Average405.556Da
Monoisotopic405.264647871Da
BASm0000274aldehydo-D-galacturonateC6H9O7Chemical structure of aldehydo-D-galacturonateNot available
Average193.132Da
Monoisotopic193.0353762Da
BASm0000387(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6Chemical structure of (6R)-5,10-methylene-5,6,7,8-tetrahydrofolateNot available
Average455.432Da
Monoisotopic455.1564286Da
BASm00004283-oxoadipateC6H6O5Chemical structure of 3-oxoadipateNot available
Average158.11Da
Monoisotopic158.022620453Da
BASm0000491D-erythruloseC4H8O4Chemical structure of D-erythruloseNot available
Average120.104Da
Monoisotopic120.0422587Da
BASm00005166-deoxyerythronolide BC21H38O6Chemical structure of 6-deoxyerythronolide BNot available
Average386.5228Da
Monoisotopic386.2668389Da

Displaying 1–10 of 1760 metabolites