Brevibacillus brevis NBRC 100599

Brevibacillus brevis NBRC 100599, formerly known as Bacillus brevis 47, is a strictly aerobic, Gram-positive, spore-forming soil bacterium. It has a characteristic three-layered cell wall composed of a peptidoglycan-layer and the outer two S-layers, each consisting of single protein species, and is known to secrete large amounts of proteins into the culture media. The proteins secreted by this strain are mainly derived from the outer two layers of the cell wall, which are shed into the medium with the progress of cell growth. In secretes little protease, which enables easy recovery of the secreted proteins with minimal degradation, and thus it has been used as a good host for production of heterologous proteins. The genome lacks several sugar metabolism genes found in Bacillus subtilis, showing that B. brevis is unable to utilize L-arabinose, D-mannose and D-xylose. The abundance of genes involved in response to environmental stress, such as those encoding sigma factors and chemotaxis receptors, suggests that this bacterium has a great capacity of adaptation to diverse environments (adapted from http://www.bio.nite.go.jp/dogan/About?GENOME_ID=bbr_G1). (HAMAP: BREBN)