Pseudomonas putida DOT-T1E

Gram-negativeRodMotileFacultative

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Gammaproteobacteria

Order

Pseudomonadales

Family

Pseudomonadaceae

Genus

Pseudomonas

Description

Pseudomonas putida DOT-T1E is a Gram-negative, rod-shaped bacterium characterized by its mesophilic temperature range and facultative anaerobic metabolism. This organism is a heterotroph, thriving in environments such as soil and wastewater, where it plays a significant role in nutrient cycling and organic matter decomposition. P. putida DOT-T1E has a single cell arrangement and exhibits mobility, facilitated by the presence of flagella. Its structural characteristics include two membranes, which is typical for Gram-negative bacteria, and a single replicon, indicating a streamlined genetic organization. As a free-living bacterium, P. putida DOT-T1E contributes to the biogeochemical processes within its habitats, such as the degradation of pollutants and organic compounds. Its adaptability to various environmental conditions underscores its ecological importance, particularly in wastewater treatment systems where it can help mitigate the impact of contaminants. Overall, Pseudomonas putida DOT-T1E exemplifies the functional diversity of microbial life and its potential applications in bioremediation and environmental sustainability.

Taxonomy

Kingdom	Pseudomonadati
Phylum	Pseudomonadota
Class	Gammaproteobacteria
Order	Pseudomonadales
Family	Pseudomonadaceae
Genus	Pseudomonas
Species	Pseudomonas putida
Strain	DOT-T1E

Profile

Physiology
Gram staining properties	Negative
Shape	Rod
Mobility	Yes
Flagellar presence	Yes
Number of membranes	2

Image source: Wikipedia/Wikimedia

Ecology, Host, and Life Cycle
Oxygen requirements	Facultative
Optimal temperature	Not Available
Temperature range	Mesophilic
Habitat	Soil - Wastewater
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Singles
Sporulation	Not Available
Energy source	Heterotroph
Pathogenicity	Not Available

Genome Summary

Pseudomonas putida DOT-T1E, complete sequence.

NC_018220.1

Gene Summary

Adenine Count

1201150 bp

Thymine Count

1213598 bp

Guanine Count

1930834 bp

Cytosine Count

1915115 bp

Genome Length

6260702 bp

Protein-coding Genes

5695 genes

Non-Coding Genes

158 genes

# of Chromosomes/Plasmids

Genes

Name	Locus Tag	UniProt ID	Strand Orientation	Gene Start/End	Protein Molecular Weight
polyamine abc transporter substrate-binding protein	T1E_RS20185	Not Available	Negative	4342376 - 4343479	40637.9
histone deacetylase family protein	T1E_RS20190	Not Available	Negative	4343479 - 4344522	37878.5
arac family transcriptional regulator	T1E_RS20195	Not Available	Negative	4344819 - 4345811	36620.1
aspartate ammonia-lyase	T1E_RS20200	Not Available	Negative	4346042 - 4347466	51045.0
lysr substrate-binding domain-containing protein	T1E_RS20205	Not Available	Positive	4347649 - 4348566	33519.5
zinc-dependent metalloprotease family protein	T1E_RS20210	Not Available	Negative	4348558 - 4349184	23778.0
5-(carboxyamino)imidazole ribonucleotide mutase	T1E_RS20215	Not Available	Positive	4349455 - 4349946	16944.6
5-(carboxyamino)imidazole ribonucleotide synthase	T1E_RS20220	Not Available	Positive	4349957 - 4351039	38690.5
glsb/yeaq/ymge family stress response membrane protein	T1E_RS20225	Not Available	Positive	4351148 - 4351393	8326.72
duf3299 domain-containing protein	T1E_RS20230	Not Available	Positive	4351442 - 4351975	19229.4

Displaying genes 4031 – 4040 of 5853 in total

Pathways

6 pathways

Cymene and Cumate degradation

Naphthalene degradation

TCA Cycle

Nicotine degradation, pyrrolidine pathway

Metabolite ID	Metabolite name	Structure	CAS number	Average mass Monoisotopic mass
BASm0000253	2-oxopent-4-enoateC5H5O3		Not available	Average113.093Da Monoisotopic113.024417601Da
BASm0000599	2,5-dihydroxypyridineC5H5NO2		Not available	Average111.1Da Monoisotopic111.0320284Da
BASm0001885	Tetra-mu3-sulfido-tetrairon(1+)Fe4S4		Not available	Average351.62Da Monoisotopic351.62748Da
BASm0002000	2,3-dihydroxy-p-cumateC10H11O4		Not available	Average195.195Da Monoisotopic195.0662824Da
BASm0002146	(1R,2S)-1,2-dihydronaphthalene-1,2-diolC10H10O2		Not available	Average162.1852Da Monoisotopic162.0680796Da
BASm0003833	2-hydroxychromene-2-carboxylateC10H7O4	Not available	Not available	Average191.163Da Monoisotopic191.034982285Da
BASm0014033	AmmoniaH3N		`7664-41-7`	Average17.0305Da Monoisotopic17.026549101Da
BASm0014037	Fumaric acidC4H4O4		`110-17-8`	Average116.0722Da Monoisotopic116.010958616Da
BASm0014038	Malic acidC4H6O5		`97-67-6`	Average134.0874Da Monoisotopic134.021523302Da
BASm0014042	Oxoglutaric acidC5H6O5		`328-50-7`	Average146.0981Da Monoisotopic146.021523302Da