Pseudomonas putida DOT-T1E

Gram-negativeRodMotileFacultative

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Gammaproteobacteria

Order

Pseudomonadales

Family

Pseudomonadaceae

Genus

Pseudomonas

Description

Pseudomonas putida DOT-T1E is a Gram-negative, rod-shaped bacterium characterized by its mesophilic temperature range and facultative anaerobic metabolism. This organism is a heterotroph, thriving in environments such as soil and wastewater, where it plays a significant role in nutrient cycling and organic matter decomposition. P. putida DOT-T1E has a single cell arrangement and exhibits mobility, facilitated by the presence of flagella. Its structural characteristics include two membranes, which is typical for Gram-negative bacteria, and a single replicon, indicating a streamlined genetic organization. As a free-living bacterium, P. putida DOT-T1E contributes to the biogeochemical processes within its habitats, such as the degradation of pollutants and organic compounds. Its adaptability to various environmental conditions underscores its ecological importance, particularly in wastewater treatment systems where it can help mitigate the impact of contaminants. Overall, Pseudomonas putida DOT-T1E exemplifies the functional diversity of microbial life and its potential applications in bioremediation and environmental sustainability.

Taxonomy

KingdomPseudomonadati
PhylumPseudomonadota
ClassGammaproteobacteria
OrderPseudomonadales
FamilyPseudomonadaceae
GenusPseudomonas
SpeciesPseudomonas putida
StrainDOT-T1E

Profile

Physiology
Gram staining propertiesNegative
ShapeRod
MobilityYes
Flagellar presenceYes
Number of membranes2
Image of Pseudomonas putida DOT-T1E
Image source: Wikipedia/Wikimedia
Ecology, Host, and Life Cycle
Oxygen requirementsFacultative
Optimal temperatureNot Available
Temperature rangeMesophilic
HabitatSoil - Wastewater
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementSingles
SporulationNot Available
Energy sourceHeterotroph
PathogenicityNot Available

Genome Summary

Pseudomonas putida DOT-T1E, complete sequence.

Gene Summary

Adenine Count

1201150 bp

Thymine Count

1213598 bp

Guanine Count

1930834 bp

Cytosine Count

1915115 bp

Genome Length

6260702 bp

Protein-coding Genes

5695 genes

Non-Coding Genes

158 genes

# of Chromosomes/Plasmids

1

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
lysr family transcriptional regulatorT1E_RS17115Not AvailableNegative3728515 - 372945935444.4
yebc/pmpr family dna-binding transcriptional regulatorT1E_RS17120Not AvailableNegative3729597 - 373031025316.8
fad-dependent oxidoreductaseT1E_RS17125Not AvailableNegative3730408 - 373179949872.2
multicopper oxidase domain-containing proteinT1E_RS17130Not AvailableNegative3731880 - 373313044032.7
lysr substrate-binding domain-containing proteinT1E_RS17135Not AvailablePositive3733374 - 373424331945.5
duf2784 domain-containing proteinT1E_RS17140Not AvailablePositive3734401 - 373477213806.3
trans-aconitate 2-methyltransferaseT1E_RS17145Not AvailableNegative3734762 - 373553829083.7
hypothetical proteinT1E_RS17150Not AvailablePositive3735653 - 37358326417.85
amino acid permeaseT1E_RS17155Not AvailableNegative3735836 - 373724850989.4
plp-dependent aminotransferase family proteinT1E_RS17160Not AvailablePositive3737574 - 373899853148.2

Displaying genes 3421 – 3430 of 5853 in total

Metabolites

18 records
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Result columns

Structure search compares stored SMILES, InChI, and InChIKey text. Exact mass must match the stored database value.

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Metabolite IDMetabolite nameStructureCAS number
Average massMonoisotopic mass
BASm00002532-oxopent-4-enoateC5H5O3Chemical structure of 2-oxopent-4-enoateNot available
Average113.093Da
Monoisotopic113.024417601Da
BASm00005992,5-dihydroxypyridineC5H5NO2Chemical structure of 2,5-dihydroxypyridineNot available
Average111.1Da
Monoisotopic111.0320284Da
BASm0001885Tetra-mu3-sulfido-tetrairon(1+)Fe4S4Chemical structure of Tetra-mu3-sulfido-tetrairon(1+)Not available
Average351.62Da
Monoisotopic351.62748Da
BASm00020002,3-dihydroxy-p-cumateC10H11O4Chemical structure of 2,3-dihydroxy-p-cumateNot available
Average195.195Da
Monoisotopic195.0662824Da
BASm0002146(1R,2S)-1,2-dihydronaphthalene-1,2-diolC10H10O2Chemical structure of (1R,2S)-1,2-dihydronaphthalene-1,2-diolNot available
Average162.1852Da
Monoisotopic162.0680796Da
BASm00038332-hydroxychromene-2-carboxylateC10H7O4Not availableNot available
Average191.163Da
Monoisotopic191.034982285Da
BASm0014033AmmoniaH3NChemical structure of Ammonia7664-41-7
Average17.0305Da
Monoisotopic17.026549101Da
BASm0014037Fumaric acidC4H4O4Chemical structure of Fumaric acid110-17-8
Average116.0722Da
Monoisotopic116.010958616Da
BASm0014038Malic acidC4H6O5Chemical structure of Malic acid97-67-6
Average134.0874Da
Monoisotopic134.021523302Da
BASm0014042Oxoglutaric acidC5H6O5Chemical structure of Oxoglutaric acid328-50-7
Average146.0981Da
Monoisotopic146.021523302Da

Displaying 1–10 of 18 metabolites

Health Effects

No health effects information available for this bacterium.