Methylobacterium nodulans ORS 2060

Gram-negativeRodNon-motileAerobe

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Alphaproteobacteria

Order

Hyphomicrobiales

Family

Methylobacteriaceae

Genus

Methylobacterium

Description

In 2001 Methylobacterium, which had been hitherto found associated non-symbiotically with plants, was discovered to nodulate Crotalaria, a large Fabaceae group found in Africa. Strain ORS 2060 (also known as CNCM I 2342T=LMG 21967T), the type strain for this species, was isolated from nodules on C. podocarpa from the Bel-Air area, Dakar, Senegal. It is able to use C1 compounds such as methanol, formate and formaldehyde but not methylamine as its sole carbon source, as well as more complex carbon sources such as succinate, citrate, pyruvate, glutamate and ethanol. It grows as short asporogenous Gram-negative rods that occur singly or occasionally in pairs; some are motile with one or more polar flagella. Optimal growth occurs at pH 6.8-7.5 and at 30-37 degrees C. It is strictly aerobic and unpigmented (adapted from PubMed 15545469 and http://genome.jgi-psf.org/finished_microbes/metno/metno.home.html). (EBI Integr8)

Taxonomy

KingdomPseudomonadati
PhylumPseudomonadota
ClassAlphaproteobacteria
OrderHyphomicrobiales
FamilyMethylobacteriaceae
GenusMethylobacterium
SpeciesMethylobacterium nodulans
StrainORS 2060

Profile

Physiology
Gram staining propertiesNegative
ShapeRod
MobilityNo
Flagellar presenceYes
Number of membranes2
Image of Methylobacterium nodulans ORS 2060
Image source: Wikipedia/Wikimedia
Ecology, Host, and Life Cycle
Oxygen requirementsAerobe
Optimal temperature32
Temperature rangeMesophilic
HabitatHostAssociated
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementPairs - Singles
SporulationNonsporulating
Energy sourceMethylotroph
PathogenicityNo

Genome Summary

Methylobacterium nodulans ORS 2060 plasmid pMNOD07, complete

Gene Summary

Adenine Count

1593 bp

Thymine Count

1627 bp

Guanine Count

3320 bp

Cytosine Count

3289 bp

Genome Length

9829 bp

Protein-coding Genes

7 genes

Non-Coding Genes

0 genes

# of Chromosomes/Plasmids

5

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
anti-sigma factor family proteinMNOD_RS21115Not AvailableNegative4535616 - 453639527526.7
sigma-70 family rna polymerase sigma factorMNOD_RS21120Not AvailableNegative4536392 - 453697021451.7
gamma-glutamyltransferaseMNOD_RS21125Not AvailableNegative4537032 - 453878360080.0
lyse family translocatorMNOD_RS21130Not AvailablePositive4538900 - 453953522180.8
hypothetical proteinMNOD_RS21135Not AvailableNegative4539818 - 454033317334.8
23s rrna (adenine(2503)-c(2))-methyltransferase rlmnMNOD_RS21140Not AvailableNegative4540349 - 454164447658.1
hypothetical proteinMNOD_RS21145Not AvailableNegative4541736 - 454234721277.5
phosphotransferaseMNOD_RS21150Not AvailablePositive4542582 - 454361638204.7
hypothetical proteinMNOD_RS21155Not AvailableNegative4543666 - 45438637234.66
marr family winged helix-turn-helix transcriptional regulatorMNOD_RS21160Not AvailableNegative4544144 - 454472520921.0

Displaying genes 4791 – 4800 of 8224 in total

Metabolites

1759 records
Metabolite IDMetabolite nameStructureCAS number
BASm00002502,5-didehydro-D-gluconateC6H7O7Chemical structure of 2,5-didehydro-D-gluconate53736-12-2
Average191.1156Da
Monoisotopic191.019177578Da
BASm00002532-oxopent-4-enoateC5H5O3Chemical structure of 2-oxopent-4-enoateNot available
Average113.093Da
Monoisotopic113.024417601Da
BASm00002603alpha,7alpha-dihydroxy-12-oxo-5beta-cholanateC24H37O5Chemical structure of 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanateNot available
Average405.556Da
Monoisotopic405.264647871Da
BASm0000274aldehydo-D-galacturonateC6H9O7Chemical structure of aldehydo-D-galacturonateNot available
Average193.132Da
Monoisotopic193.0353762Da
BASm00004283-oxoadipateC6H6O5Chemical structure of 3-oxoadipateNot available
Average158.11Da
Monoisotopic158.022620453Da
BASm00005166-deoxyerythronolide BC21H38O6Chemical structure of 6-deoxyerythronolide BNot available
Average386.5228Da
Monoisotopic386.2668389Da
BASm0000553biphenyl-2,3-diolC12H10O2Chemical structure of biphenyl-2,3-diolNot available
Average186.2066Da
Monoisotopic186.0680796Da
BASm0000592(S)-1-phenylethanolC8H10OChemical structure of (S)-1-phenylethanolNot available
Average122.1644Da
Monoisotopic122.0731649Da
BASm00005992,5-dihydroxypyridineC5H5NO2Chemical structure of 2,5-dihydroxypyridineNot available
Average111.1Da
Monoisotopic111.0320284Da
BASm0000893crotonobetaineC7H13NO2Chemical structure of crotonobetaine927-89-9
Average143.1836Da
Monoisotopic143.0946287Da

Displaying 1–10 of 1759 metabolites

Health Effects

No health effects information available for this bacterium.