Geobacillus thermodenitrificans NG80-2

Geobacillus thermodenitrificans (strain NG80-2) is a facultative aerobic thermophilic bacterium phylogenetically associated with the Firmicutes. It was isolated from oilfield in Dagang (Northern China) at a depth of 2000 m and a temperature of 73 degrees Celsius. The complete genome sequence consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches (not only in geothermal areas, but also in temperate regions and permanently cold habitats), by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by in vivo and in vitro experiments. This strain can use crude oil as a sole carbon source and can degrade 16 to 36 carbon alkanes. It grows between 45 degrees Celsius and 73 degrees Celsius (optimum 65 degrees Celsius), and is capable of oxygen and nitrate respiration. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules. Geobacillus thermodenitrificans NG80-2 also produces an emulsifier, which may be useful for high temperature biodegradation or other industrial purposes. (EBI Integr8)