Clostridium novyi NT

Gram-positiveRodMotileAnaerobe

Kingdom

Bacillati

Phylum

Bacillota

Class

Clostridia

Order

Eubacteriales

Family

Clostridiaceae

Genus

Clostridium

Description

Clostridium novyi-NT, a Gram +, spore-forming bacteria, is an experimentally developed attenuated strain of pathogenic C.novyi. To do so, the major systemic toxin gene-containing episome has been deleted. It was created to be used as an bacteriolytic anti-tumor agent. An anaerobic bacterium is ideally suited as an anti-tumor agent as the only hypoxic regions within mammals are tumors. Intravenous injection of C.novyi-NT spores in rabbits and mice has produced anti-tumor effects without excessive toxicity. While the spores are very stable, the vegetative form is very sensitive to oxygen. (EBI Integr8)

Taxonomy

Kingdom	Bacillati
Phylum	Bacillota
Class	Clostridia
Order	Eubacteriales
Family	Clostridiaceae
Genus	Clostridium
Species	Clostridium novyi
Strain	NT

Profile

Physiology
Gram staining properties	Positive
Shape	Rod
Mobility	Yes
Flagellar presence	Not Available
Number of membranes	1

Ecology, Host, and Life Cycle
Oxygen requirements	Anaerobe
Optimal temperature	Not Available
Temperature range	Mesophilic
Habitat	Terrestrial
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Pairs - Singles
Sporulation	Sporulating
Energy source	Chemoorganotroph
Pathogenicity	No

Genome Summary

Clostridium novyi NT, complete sequence.

NC_008593.1

Gene Summary

Adenine Count

911949 bp

Thymine Count

900609 bp

Guanine Count

369148 bp

Cytosine Count

366014 bp

Genome Length

2547720 bp

Protein-coding Genes

2272 genes

Non-Coding Genes

167 genes

# of Chromosomes/Plasmids

Genes

Name	Locus Tag	UniProt ID	Strand Orientation	Gene Start/End	Protein Molecular Weight
Trna-phe	Not Available	Not Available	Positive	2472004 - 2472079	Not Available
5s ribosomal rna	Not Available	Not Available	Positive	2472085 - 2472201	Not Available
23s ribosomal rna	Not Available	Not Available	Positive	2472238 - 2475142	Not Available
16s ribosomal rna	Not Available	Not Available	Positive	2475317 - 2476829	Not Available
cpbp family intramembrane glutamic endopeptidase	NT01CX_RS11525	Not Available	Negative	2477178 - 2478071	33780.5
abc transporter permease	NT01CX_RS11530	Not Available	Negative	2478197 - 2478979	29215.0
abc transporter atp-binding protein	NT01CX_RS11535	Not Available	Negative	2478979 - 2479674	26326.1
gntr family transcriptional regulator	NT01CX_RS11540	Not Available	Negative	2479677 - 2480045	14138.4
paai family thioesterase	NT01CX_RS11545	Not Available	Negative	2480185 - 2480601	15490.9
uracil-dna glycosylase	NT01CX_RS11550	Not Available	Positive	2480771 - 2481367	22586.2

Displaying genes 2371 – 2380 of 2439 in total

Pathways

1117 pathways

2,3-Dihydroxybenzoate Biosynthesis

Biosynthesis of Siderophore Group Nonribosomal Peptides

Lysine Biosynthesis

Glycolysis and Pyruvate Dehydrogenase

Arginine Metabolism

Ornithine Metabolism

Palmitate Biosynthesis

Fatty Acid Elongation (Saturated)

Show All 1117 Pathways

Metabolite ID	Metabolite name	CAS number	Average mass Monoisotopic mass
BASm0000217	alpha-ribazoleC14H18N2O4	Not available	Average278.3037Da Monoisotopic278.126657074Da
BASm0000237	(R)-4'-phosphopantothenateC9H18NO8P	Not available	Average299.2149Da Monoisotopic299.0770031Da
BASm0000251	2-dehydropantoateC6H9O4	Not available	Average145.1333Da Monoisotopic145.050083776Da
BASm0000387	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6	Not available	Average455.432Da Monoisotopic455.1564286Da
BASm0001717	fumarateC4H2O4	Not available	Average114.0563Da Monoisotopic113.9953086Da
BASm0001865	diphosphateHO7P2	Not available	Average174.95Da Monoisotopic174.9213971Da
BASm0001921	(S)-3-methyl-2-oxopentanoateC6H9O3	`1460-34-0`	Average129.1339Da Monoisotopic129.0551692Da
BASm0002157	7-cyano-7-deazaguanineC7H5N5O	Not available	Average175.1475Da Monoisotopic175.0494098Da
BASm0002198	beta-D-ribofuranoseC5H10O5	`50-69-1`	Average150.1299Da Monoisotopic150.05282343Da
BASm0002305	(S)-2-ethyl-2-hydroxy-3-oxobutanoateC6H9O4	Not available	Average145.135Da Monoisotopic145.0506324Da

Displaying 1–10 of 121 metabolites

Health Effects

No health effects information available for this bacterium.