Clostridium novyi NT

Gram-positiveRodMotileAnaerobe

Kingdom

Bacillati

Phylum

Bacillota

Class

Clostridia

Order

Eubacteriales

Family

Clostridiaceae

Genus

Clostridium

Description

Clostridium novyi-NT, a Gram +, spore-forming bacteria, is an experimentally developed attenuated strain of pathogenic C.novyi. To do so, the major systemic toxin gene-containing episome has been deleted. It was created to be used as an bacteriolytic anti-tumor agent. An anaerobic bacterium is ideally suited as an anti-tumor agent as the only hypoxic regions within mammals are tumors. Intravenous injection of C.novyi-NT spores in rabbits and mice has produced anti-tumor effects without excessive toxicity. While the spores are very stable, the vegetative form is very sensitive to oxygen. (EBI Integr8)

Taxonomy

Kingdom	Bacillati
Phylum	Bacillota
Class	Clostridia
Order	Eubacteriales
Family	Clostridiaceae
Genus	Clostridium
Species	Clostridium novyi
Strain	NT

Profile

Physiology
Gram staining properties	Positive
Shape	Rod
Mobility	Yes
Flagellar presence	Not Available
Number of membranes	1

Ecology, Host, and Life Cycle
Oxygen requirements	Anaerobe
Optimal temperature	Not Available
Temperature range	Mesophilic
Habitat	Terrestrial
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Pairs - Singles
Sporulation	Sporulating
Energy source	Chemoorganotroph
Pathogenicity	No

Genome Summary

Clostridium novyi NT, complete sequence.

NC_008593.1

Gene Summary

Adenine Count

911949 bp

Thymine Count

900609 bp

Guanine Count

369148 bp

Cytosine Count

366014 bp

Genome Length

2547720 bp

Protein-coding Genes

2272 genes

Non-Coding Genes

167 genes

# of Chromosomes/Plasmids

Genes

Name	Locus Tag	UniProt ID	Strand Orientation	Gene Start/End	Protein Molecular Weight
sensor histidine kinase	NT01CX_RS11115	Not Available	Positive	2390901 - 2392292	52055.0
hypothetical protein	NT01CX_RS11120	Not Available	Positive	2392383 - 2393936	58862.2
polysaccharide deacetylase family protein	NT01CX_RS11125	Not Available	Positive	2393961 - 2394887	33888.5
uracil-dna glycosylase family protein	NT01CX_RS11130	Not Available	Negative	2394967 - 2395524	21508.4
ig-like domain-containing protein	NT01CX_RS11135	Not Available	Positive	2395671 - 2397260	61501.8
is1182-like element iscno1 family transposase	NT01CX_RS11140	Not Available	Negative	2397317 - 2398755	56666.4
mate family efflux transporter	NT01CX_RS11145	Not Available	Positive	2399031 - 2400383	49412.5
homoserine kinase	NT01CX_RS11150	Not Available	Negative	2400425 - 2401324	33452.3
hypothetical protein	NT01CX_RS11155	Not Available	Negative	2401528 - 2401722	7052.78
spore maturation protein	NT01CX_RS11160	Not Available	Negative	2401833 - 2402357	18953.2

Displaying genes 2291 – 2300 of 2439 in total

Pathways

1117 pathways

2,3-Dihydroxybenzoate Biosynthesis

Biosynthesis of Siderophore Group Nonribosomal Peptides

Lysine Biosynthesis

Glycolysis and Pyruvate Dehydrogenase

Arginine Metabolism

Ornithine Metabolism

Palmitate Biosynthesis

Fatty Acid Elongation (Saturated)

Show All 1117 Pathways

Metabolite ID	Metabolite name	CAS number	Average mass Monoisotopic mass
BASm0000217	alpha-ribazoleC14H18N2O4	Not available	Average278.3037Da Monoisotopic278.126657074Da
BASm0000237	(R)-4'-phosphopantothenateC9H18NO8P	Not available	Average299.2149Da Monoisotopic299.0770031Da
BASm0000251	2-dehydropantoateC6H9O4	Not available	Average145.1333Da Monoisotopic145.050083776Da
BASm0000387	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6	Not available	Average455.432Da Monoisotopic455.1564286Da
BASm0001717	fumarateC4H2O4	Not available	Average114.0563Da Monoisotopic113.9953086Da
BASm0001865	diphosphateHO7P2	Not available	Average174.95Da Monoisotopic174.9213971Da
BASm0001921	(S)-3-methyl-2-oxopentanoateC6H9O3	`1460-34-0`	Average129.1339Da Monoisotopic129.0551692Da
BASm0002157	7-cyano-7-deazaguanineC7H5N5O	Not available	Average175.1475Da Monoisotopic175.0494098Da
BASm0002198	beta-D-ribofuranoseC5H10O5	`50-69-1`	Average150.1299Da Monoisotopic150.05282343Da
BASm0002305	(S)-2-ethyl-2-hydroxy-3-oxobutanoateC6H9O4	Not available	Average145.135Da Monoisotopic145.0506324Da

Displaying 1–10 of 121 metabolites

Health Effects

No health effects information available for this bacterium.