Methanocella paludicola SANAE

RodNon-motileAnaerobic

Kingdom

Methanobacteriati

Phylum

Methanobacteriota

Class

Methanomicrobia

Order

Methanocellales

Family

Methanocellaceae

Genus

Methanocella

Description

Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE) is a mesophilic, hydrogenotrophic methanogen archaeon isolated from an anaerobic, propionate-degrading enrichment culture, which was originally established from rice paddy soil. It belongs to an abundant and ubiquitous group of methanogens called rice cluster I (RC-I) whose members are most active and play a key role in methane production from rice paddy field (RPF). Growth of M. paludicola is observed at 25-40 degrees Celsius, with an optimum temperature range for growth of 35-37 degrees Celsius. The pH range for growth is 6.5-7.8, with an optimum at pH 7.0. It is able to utilize H2/CO2 and formate for growth and methane production. M. paludicola possesses the mcrA gene encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane production pathway. (Adaptated from PMID: 18398197). (HAMAP: METPS)

Taxonomy

KingdomMethanobacteriati
PhylumMethanobacteriota
ClassMethanomicrobia
OrderMethanocellales
FamilyMethanocellaceae
GenusMethanocella
SpeciesMethanocella paludicola
StrainSANAE

Profile

Physiology
Gram staining propertiesNot Available
ShapeRod
MobilityNo
Flagellar presenceYes
Number of membranes1
Ecology, Host, and Life Cycle
Oxygen requirementsAnaerobic
Optimal temperature37
Temperature rangeMesophilic
HabitatNot Available
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementSingles
SporulationNot Available
Energy sourceNot Available
PathogenicityNo

Genome Summary

Methanocella paludicola SANAE, complete sequence.

Gene Summary

Adenine Count

665167 bp

Thymine Count

668227 bp

Guanine Count

811830 bp

Cytosine Count

812411 bp

Genome Length

2957635 bp

Protein-coding Genes

3048 genes

Non-Coding Genes

57 genes

# of Chromosomes/Plasmids

1

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
triose-phosphate isomeraseMCP_RS14710Not AvailableNegative2853815 - 285447123659.7
nadph-dependent fmn reductaseMCP_RS14715Not AvailablePositive2854531 - 285508820740.0
bifunctional 5,6,7,8-tetrahydromethanopterin hydro-lyase/3-hexulose-6-phosphate synthaseMCP_RS14720Not AvailableNegative2855120 - 285631042682.3
winged helix-turn-helix transcriptional regulatorMCP_RS14725Not AvailableNegative2856552 - 285739430742.5
hypothetical proteinMCP_RS14730Not AvailablePositive2857602 - 285803916434.9
tryptophan synthase subunit alphaMCP_RS14735Not AvailableNegative2858014 - 285884429001.1
tryptophan synthase subunit betaMCP_RS14740Not AvailableNegative2858841 - 286001942642.1
phosphoribosylanthranilate isomeraseMCP_RS14745Not AvailableNegative2860006 - 286065022398.3
anthranilate phosphoribosyltransferaseMCP_RS14750Not AvailableNegative2860647 - 286169036385.1
anthranilate synthase component iiMCP_RS14755Not AvailableNegative2861691 - 286226020521.9

Displaying genes 3001 – 3010 of 3105 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolites

4 records
Metabolite IDMetabolite nameStructureCAS number
BASm0001865diphosphateHO7P2Chemical structure of diphosphateNot available
Average174.95Da
Monoisotopic174.9213971Da
BASm0003389NADP(+)C21H25N7O17P3Chemical structure of NADP(+)Not available
Average740.386Da
Monoisotopic740.053624107Da
BASm0003878(2S)-2-phospholactateC3H4O6PChemical structure of (2S)-2-phospholactateNot available
Average167.034Da
Monoisotopic166.976195587Da
BASm0017271NADC21H28N7O14P2Chemical structure of NAD53-84-9
Average664.433Da
Monoisotopic664.116946663Da

Displaying 1–4 of 4 metabolites

Health Effects

No health effects information available for this bacterium.