Methanocella paludicola SANAE

RodNon-motileAnaerobic

Kingdom

Methanobacteriati

Phylum

Methanobacteriota

Class

Methanomicrobia

Order

Methanocellales

Family

Methanocellaceae

Genus

Methanocella

Description

Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE) is a mesophilic, hydrogenotrophic methanogen archaeon isolated from an anaerobic, propionate-degrading enrichment culture, which was originally established from rice paddy soil. It belongs to an abundant and ubiquitous group of methanogens called rice cluster I (RC-I) whose members are most active and play a key role in methane production from rice paddy field (RPF). Growth of M. paludicola is observed at 25-40 degrees Celsius, with an optimum temperature range for growth of 35-37 degrees Celsius. The pH range for growth is 6.5-7.8, with an optimum at pH 7.0. It is able to utilize H2/CO2 and formate for growth and methane production. M. paludicola possesses the mcrA gene encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane production pathway. (Adaptated from PMID: 18398197). (HAMAP: METPS)

Taxonomy

Kingdom	Methanobacteriati
Phylum	Methanobacteriota
Class	Methanomicrobia
Order	Methanocellales
Family	Methanocellaceae
Genus	Methanocella
Species	Methanocella paludicola
Strain	SANAE

Profile

Physiology
Gram staining properties	Not Available
Shape	Rod
Mobility	No
Flagellar presence	Yes
Number of membranes	1

Ecology, Host, and Life Cycle
Oxygen requirements	Anaerobic
Optimal temperature	37
Temperature range	Mesophilic
Habitat	Not Available
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Singles
Sporulation	Not Available
Energy source	Not Available
Pathogenicity	No

Genome Summary

Methanocella paludicola SANAE, complete sequence.

NC_013665.1

Gene Summary

Adenine Count

665167 bp

Thymine Count

668227 bp

Guanine Count

811830 bp

Cytosine Count

812411 bp

Genome Length

2957635 bp

Protein-coding Genes

3048 genes

Non-Coding Genes

57 genes

# of Chromosomes/Plasmids

Genes

Name	Locus Tag	UniProt ID	Strand Orientation	Gene Start/End	Protein Molecular Weight
gdp-l-fucose synthase family protein	MCP_RS13785	Not Available	Negative	2667439 - 2668377	35216.2
gdp-mannose 4,6-dehydratase	MCP_RS13790	Not Available	Negative	2668377 - 2669435	40117.5
dtdp-4-dehydrorhamnose 3,5-epimerase family protein	MCP_RS13795	Not Available	Positive	2669703 - 2670182	18502.3
dtdp-glucose 4,6-dehydratase	MCP_RS13800	Not Available	Positive	2670188 - 2671147	35876.9
glucose-1-phosphate thymidylyltransferase	MCP_RS13805	Not Available	Negative	2671155 - 2672228	38414.5
dtdp-4-dehydrorhamnose reductase	MCP_RS13810	Not Available	Negative	2672225 - 2673070	30984.9
udp-glucuronic acid decarboxylase family protein	MCP_RS13815	Not Available	Negative	2673067 - 2674026	35562.5
sugar phosphate nucleotidyltransferase	MCP_RS13820	Not Available	Negative	2674038 - 2675042	36362.6
pqq-binding-like beta-propeller repeat protein	MCP_RS13825	Not Available	Negative	2675055 - 2676254	42013.9
oligosaccharyl transferase, archaeosortase a system-associated	MCP_RS13830	Not Available	Negative	2676251 - 2678701	87948.3

Displaying genes 2811 – 2820 of 3105 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolite ID	Metabolite name	Structure	CAS number	Average mass Monoisotopic mass
BASm0001865	diphosphateHO7P2		Not available	Average174.95Da Monoisotopic174.9213971Da
BASm0003389	NADP(+)C21H25N7O17P3		Not available	Average740.386Da Monoisotopic740.053624107Da
BASm0003878	(2S)-2-phospholactateC3H4O6P		Not available	Average167.034Da Monoisotopic166.976195587Da
BASm0017271	NADC21H28N7O14P2		`53-84-9`	Average664.433Da Monoisotopic664.116946663Da
BASm0040062	Acinetobacter baumannii ATCC 17978	Not available	Not available	Not available
BASm0040295	Archaeoglobus profundus DSM 5631	Not available	Not available	Not available

Displaying 1–6 of 6 metabolites

Health Effects

No health effects information available for this bacterium.