Methanocella paludicola SANAE

RodNon-motileAnaerobic

Kingdom

Methanobacteriati

Phylum

Methanobacteriota

Class

Methanomicrobia

Order

Methanocellales

Family

Methanocellaceae

Genus

Methanocella

Description

Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE) is a mesophilic, hydrogenotrophic methanogen archaeon isolated from an anaerobic, propionate-degrading enrichment culture, which was originally established from rice paddy soil. It belongs to an abundant and ubiquitous group of methanogens called rice cluster I (RC-I) whose members are most active and play a key role in methane production from rice paddy field (RPF). Growth of M. paludicola is observed at 25-40 degrees Celsius, with an optimum temperature range for growth of 35-37 degrees Celsius. The pH range for growth is 6.5-7.8, with an optimum at pH 7.0. It is able to utilize H2/CO2 and formate for growth and methane production. M. paludicola possesses the mcrA gene encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane production pathway. (Adaptated from PMID: 18398197). (HAMAP: METPS)

Taxonomy

Kingdom	Methanobacteriati
Phylum	Methanobacteriota
Class	Methanomicrobia
Order	Methanocellales
Family	Methanocellaceae
Genus	Methanocella
Species	Methanocella paludicola
Strain	SANAE

Profile

Physiology
Gram staining properties	Not Available
Shape	Rod
Mobility	No
Flagellar presence	Yes
Number of membranes	1

Ecology, Host, and Life Cycle
Oxygen requirements	Anaerobic
Optimal temperature	37
Temperature range	Mesophilic
Habitat	Not Available
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Singles
Sporulation	Not Available
Energy source	Not Available
Pathogenicity	No

Genome Summary

Methanocella paludicola SANAE, complete sequence.

NC_013665.1

Gene Summary

Adenine Count

665167 bp

Thymine Count

668227 bp

Guanine Count

811830 bp

Cytosine Count

812411 bp

Genome Length

2957635 bp

Protein-coding Genes

3048 genes

Non-Coding Genes

57 genes

# of Chromosomes/Plasmids

Genes

Name	Locus Tag	UniProt ID	Strand Orientation	Gene Start/End	Protein Molecular Weight
protease inhibitor i42 family protein	MCP_RS11000	Not Available	Positive	2151244 - 2151966	25169.9
coenzyme f420 hydrogenase/dehydrogenase, beta subunit c-terminal domain	MCP_RS11005	Not Available	Positive	2152085 - 2153110	36596.5
hypothetical protein	MCP_RS11010	Not Available	Negative	2153678 - 2154304	23878.1
metallophosphoesterase	MCP_RS11015	Not Available	Positive	2154481 - 2155275	30258.2
thioredoxin family protein	MCP_RS11020	Not Available	Negative	2155256 - 2155714	16453.7
hypothetical protein	MCP_RS11025	Not Available	Positive	2155777 - 2156079	11711.0
class i sam-dependent methyltransferase	MCP_RS11030	Not Available	Positive	2156081 - 2156659	20956.2
class ii glutamine amidotransferase	MCP_RS11035	Not Available	Positive	2156712 - 2157410	26203.8
hypothetical protein	MCP_RS11040	Not Available	Negative	2157415 - 2157636	7772.45
hypothetical protein	MCP_RS11045	Not Available	Positive	2157818 - 2158249	16163.5

Displaying genes 2251 – 2260 of 3105 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolite ID	Metabolite name	CAS number	Average mass Monoisotopic mass
BASm0001865	diphosphateHO7P2	Not available	Average174.95Da Monoisotopic174.9213971Da
BASm0003389	NADP(+)C21H25N7O17P3	Not available	Average740.386Da Monoisotopic740.053624107Da
BASm0003878	(2S)-2-phospholactateC3H4O6P	Not available	Average167.034Da Monoisotopic166.976195587Da
BASm0017271	NADC21H28N7O14P2	`53-84-9`	Average664.433Da Monoisotopic664.116946663Da

Displaying 1–4 of 4 metabolites

Health Effects

No health effects information available for this bacterium.