Methanocella paludicola SANAE

RodNon-motileAnaerobic

Kingdom

Methanobacteriati

Phylum

Methanobacteriota

Class

Methanomicrobia

Order

Methanocellales

Family

Methanocellaceae

Genus

Methanocella

Description

Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE) is a mesophilic, hydrogenotrophic methanogen archaeon isolated from an anaerobic, propionate-degrading enrichment culture, which was originally established from rice paddy soil. It belongs to an abundant and ubiquitous group of methanogens called rice cluster I (RC-I) whose members are most active and play a key role in methane production from rice paddy field (RPF). Growth of M. paludicola is observed at 25-40 degrees Celsius, with an optimum temperature range for growth of 35-37 degrees Celsius. The pH range for growth is 6.5-7.8, with an optimum at pH 7.0. It is able to utilize H2/CO2 and formate for growth and methane production. M. paludicola possesses the mcrA gene encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane production pathway. (Adaptated from PMID: 18398197). (HAMAP: METPS)

Taxonomy

Kingdom	Methanobacteriati
Phylum	Methanobacteriota
Class	Methanomicrobia
Order	Methanocellales
Family	Methanocellaceae
Genus	Methanocella
Species	Methanocella paludicola
Strain	SANAE

Profile

Physiology
Gram staining properties	Not Available
Shape	Rod
Mobility	No
Flagellar presence	Yes
Number of membranes	1

Ecology, Host, and Life Cycle
Oxygen requirements	Anaerobic
Optimal temperature	37
Temperature range	Mesophilic
Habitat	Not Available
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Singles
Sporulation	Not Available
Energy source	Not Available
Pathogenicity	No

Genome Summary

Methanocella paludicola SANAE, complete sequence.

NC_013665.1

Gene Summary

Adenine Count

665167 bp

Thymine Count

668227 bp

Guanine Count

811830 bp

Cytosine Count

812411 bp

Genome Length

2957635 bp

Protein-coding Genes

3048 genes

Non-Coding Genes

57 genes

# of Chromosomes/Plasmids

Genes

Name	Locus Tag	UniProt ID	Strand Orientation	Gene Start/End	Protein Molecular Weight
lrp/asnc ligand binding domain-containing protein	MCP_RS09705	Not Available	Negative	1926205 - 1926435	8345.2
duf5788 family protein	MCP_RS09710	Not Available	Positive	1926696 - 1927193	19297.4
hypothetical protein	MCP_RS09715	Not Available	Negative	1927221 - 1927508	11412.7
duf523 domain-containing protein	MCP_RS09720	Not Available	Negative	1927976 - 1929150	41603.4
methyl coenzyme m reductase-arginine methyltransferase mmp10	MCP_RS09725	Not Available	Negative	1929416 - 1930714	47498.1
type iv pilin	MCP_RS09730	Not Available	Negative	1930838 - 1931224	13634.4
glycosyltransferase family 2 protein	MCP_RS09735	Not Available	Positive	1931546 - 1932523	35425.2
glycosyltransferase family 2 protein	MCP_RS09740	Not Available	Negative	1932534 - 1933598	40715.5
glycosyltransferase family 4 protein	MCP_RS09745	Not Available	Positive	1933712 - 1934836	43555.1
glycosyltransferase family 2 protein	MCP_RS09750	Not Available	Negative	1934846 - 1935745	34839.7

Displaying genes 1991 – 2000 of 3105 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolite ID	Metabolite name	Structure	CAS number	Average mass Monoisotopic mass
BASm0001865	diphosphateHO7P2		Not available	Average174.95Da Monoisotopic174.9213971Da
BASm0003389	NADP(+)C21H25N7O17P3		Not available	Average740.386Da Monoisotopic740.053624107Da
BASm0003878	(2S)-2-phospholactateC3H4O6P		Not available	Average167.034Da Monoisotopic166.976195587Da
BASm0017271	NADC21H28N7O14P2		`53-84-9`	Average664.433Da Monoisotopic664.116946663Da
BASm0039655	Achromobacter xylosoxidans A8	Not available	Not available	Not available
BASm0039764	Bacteroides eggerthii	Not available	Not available	Not available
BASm0039765	Burkholderiales bacterium 1_1_47	Not available	Not available	Not available
BASm0040062	Acinetobacter baumannii ATCC 17978	Not available	Not available	Not available
BASm0040295	Archaeoglobus profundus DSM 5631	Not available	Not available	Not available

Displaying 1–9 of 9 metabolites

Health Effects

No health effects information available for this bacterium.