Aquifex aeolicus VF5

Gram-negativeCocciNon-motileAerobe

Kingdom

Pseudomonadati

Phylum

Aquificota

Class

Aquificia

Order

Aquificales

Family

Aquificaceae

Genus

Aquifex

Description

Aquifex aeolicus, which was first found in Yellowstone National Park, can grow at 96 degrees Celsius and is one of the most extreme thermophilic bacteria known. Because of this, Aquifex is thought to be one of the earliest bacteria to diverge from eubacteria. Hyperthermophilic bacteria such as Aquifex are important for industrial processes and its genes can be used in a variety of biotechnological applications.Even though Aquifex has the ability to survive at extreme temperatures, there are only a few specific heat-resistance indicators evident in the A. aeolicus genome. The genome, which is 1,551,335 bp in length, is densely packed and contains genes that overlap others. In addition, no introns or protein splicing elements have been found. This, along with a reduced metabolic flexability, is probably due to the limited genome size; the genome of this complex organism is ony one-third of the E. coli genome.Aquifex are nonsporeforming, gram-negative, generally rod-shaped organisms. They are about 2.0-6.0 micrometers in length and have a diameter of 0.4-0.5 micrometers. As autotrophic organisms, Aquifex fix carbon dioxide from the environment to get the carbon that they need. They are chemolithotrophic, which means that they draw energy for biosynthesis from inorganic chemical sources.Aquifex, meaning "water-maker," got its name because the the final product of this reaction is water. Even so, most Aquificales can use thiosulfate or sulfur as an energy source (much like chlorobium and other green sulfur bacteria) and produce sulfuric acid and H2S instead of water. Although most Aquifcales are strictly aerobic, A. pyrophilus was shown to be able to grow anaerobically by reducing nitrogen instead of oxygen (forming an end product of N2 instead of water).As a hyperthermophilic bactertium, Aquifex aeolicus grows in extremely hot tempuratures such as near volcanoes or hot springs. It needs oxygen to carry on its metabolic machinery, but it can function in relatively low levels of oxygen (A. pyrophilus can grow in levels of oxygen as low as 7.5 ppm). A. aeolicus can grow on hydrogen, oxygen, carbon dioxide, and mineral salts (Deckert et al. 1998). Aquifex species generally form large cell aggregates, which can be comprised of up to 100 individual cells.(From http://microbewiki.kenyon.edu/index.php/Aquifex) (MicrobeWiki: Aquifex)

Taxonomy

KingdomPseudomonadati
PhylumAquificota
ClassAquificia
OrderAquificales
FamilyAquificaceae
GenusAquifex
SpeciesAquifex aeolicus
StrainVF5

Profile

Physiology
Gram staining propertiesNegative
ShapeCocci
MobilityNo
Flagellar presenceYes
Number of membranes2
Image of Aquifex aeolicus VF5
Image source: Wikipedia/Wikimedia
Ecology, Host, and Life Cycle
Oxygen requirementsAerobe
Optimal temperature96
Temperature rangeHyperthermophilic
HabitatSpecialized
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementNot Available
SporulationNot Available
Energy sourceChemolithoautotroph
PathogenicityNo

Genome Summary

Aquifex aeolicus VF5, complete sequence.

Gene Summary

Adenine Count

Not Available

Thymine Count

Not Available

Guanine Count

Not Available

Cytosine Count

Not Available

Genome Length

Not Available

Protein-coding Genes

1712 genes

Non-Coding Genes

52 genes

# of Chromosomes/Plasmids

2

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
30s ribosomal protein s6AQ_RS00250Not AvailablePositive40404 - 4074513840.7
single-stranded dna-binding proteinAQ_RS00255Not AvailablePositive40735 - 4117817133.2
30s ribosomal protein s18AQ_RS00260Not AvailablePositive41197 - 414158712.85
twin-arginine translocase tata/tate family subunitAQ_RS00265Not AvailablePositive41470 - 416496545.19
twin-arginine translocase tata/tate family subunitAQ_RS00270Not AvailablePositive41650 - 418838528.44
trna (guanosine(46)-n7)-methyltransferase trmbAQ_RS00275Not AvailableNegative41880 - 4277335640.5
calcium/sodium antiporterAQ_RS00280Not AvailablePositive42795 - 4376334257.6
4fe-4s dicluster domain-containing proteinAQ_RS00285Not AvailableNegative43756 - 4426819377.9
yggt family proteinAQ_RS00290Not AvailableNegative44265 - 445019257.08
50s ribosomal protein l17AQ_RS00295Not AvailableNegative44501 - 4485713911.1

Displaying genes 51 – 60 of 1819 in total

Metabolites

1675 records
Metabolite IDMetabolite nameStructureCAS number
BASm0000237(R)-4'-phosphopantothenateC9H18NO8PChemical structure of (R)-4'-phosphopantothenateNot available
Average299.2149Da
Monoisotopic299.0770031Da
BASm00002502,5-didehydro-D-gluconateC6H7O7Chemical structure of 2,5-didehydro-D-gluconate53736-12-2
Average191.1156Da
Monoisotopic191.019177578Da
BASm00002512-dehydropantoateC6H9O4Chemical structure of 2-dehydropantoateNot available
Average145.1333Da
Monoisotopic145.050083776Da
BASm00002532-oxopent-4-enoateC5H5O3Chemical structure of 2-oxopent-4-enoateNot available
Average113.093Da
Monoisotopic113.024417601Da
BASm00002603alpha,7alpha-dihydroxy-12-oxo-5beta-cholanateC24H37O5Chemical structure of 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanateNot available
Average405.556Da
Monoisotopic405.264647871Da
BASm0000377(S)-malateC4H4O5Chemical structure of (S)-malateNot available
Average132.0716Da
Monoisotopic132.005873238Da
BASm0000387(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6Chemical structure of (6R)-5,10-methylene-5,6,7,8-tetrahydrofolateNot available
Average455.432Da
Monoisotopic455.1564286Da
BASm00005166-deoxyerythronolide BC21H38O6Chemical structure of 6-deoxyerythronolide BNot available
Average386.5228Da
Monoisotopic386.2668389Da
BASm0000553biphenyl-2,3-diolC12H10O2Chemical structure of biphenyl-2,3-diolNot available
Average186.2066Da
Monoisotopic186.0680796Da
BASm0000592(S)-1-phenylethanolC8H10OChemical structure of (S)-1-phenylethanolNot available
Average122.1644Da
Monoisotopic122.0731649Da

Displaying 1–10 of 1675 metabolites

Health Effects

No health effects information available for this bacterium.