Geobacillus stearothermophilus

RodAerobe

Kingdom

Bacillati

Phylum

Bacillota

Class

Bacilli

Order

Caryophanales

Family

Anoxybacillaceae

Genus

Geobacillus

Description

Geobacillus stearothermophilus is a gram-positive, rod-shaped bacterium classified as a thermophile, thriving optimally at elevated temperatures, typically around 55-75°C. This microbe is a chemoheterotroph, obtaining its energy through the decomposition of organic compounds, and it is an obligate aerobe, requiring oxygen for its growth and metabolism.As a member of the Bacillaceae family, G. stearothermophilus showcases characteristic resilience; it forms durable endospores that allow it to survive extreme conditions, including high heat and desiccation. These spores can withstand autoclaving, a feature that makes this organism particularly significant in sterilization validation processes in laboratories and healthcare settings. The ability to thrive in such harsh environments also points to its widespread presence in soil and thermal springs, where it plays a crucial role in the decomposition of organic matter. In laboratory and industrial applications, G. stearothermophilus is often used as a biological indicator to assess the effectiveness of sterilization processes, particularly in the medical field. Its spores serve as a benchmark; if the spores are killed during sterilization, it provides confidence that other pathogens have also been eliminated.Furthermore, G. stearothermophilus is notable for producing thermostable enzymes, which are utilized in various biotechnological applications, such as biofuel production and food processing, due to their activity at high temperatures. This bacterium’s ability to thrive under extreme conditions and its practical applications in industry highlight its significance within the microbial world.

Taxonomy

KingdomBacillati
PhylumBacillota
ClassBacilli
OrderCaryophanales
FamilyAnoxybacillaceae
GenusGeobacillus
SpeciesGeobacillus stearothermophilus
StrainNo strain

Profile

Physiology
Gram staining propertiesVariable
ShapeRod
MobilityNot Available
Flagellar presenceYes
Number of membranesNot Available
Image of Geobacillus stearothermophilus
Image source: Wikipedia/Wikimedia
Ecology, Host, and Life Cycle
Oxygen requirementsAerobe
Optimal temperatureNot Available
Temperature rangethermophilic
Habitatactive volcanic area; spoiled canned food
Biotic relationshipNot Available
Host(s)Not Available
Cell arrangementNot Available
SporulationNot Available
Energy sourceNot Available
PathogenicityNot Available

Genome Summary

Geobacillus stearothermophilus strain C1BS50MT1

Gene Summary

Adenine Count

798575 bp

Thymine Count

783320 bp

Guanine Count

857872 bp

Cytosine Count

861680 bp

Genome Length

3301447 bp

Protein-coding Genes

3255 genes

Non-Coding Genes

102 genes

# of Chromosomes/Plasmids

2

Genes

NameLocus TagUniProt IDStrand OrientationGene Start/EndProtein Molecular Weight
high-affinity zinc uptake system binding-protein znuaAVP43_01301Not AvailableNegative1284736 - 128570136425.8
putative membrane protein insertion efficiency factorAVP43_01302Q5KVY7Negative1285910 - 12861499068.46
s-ribosylhomocysteine lyaseAVP43_01303Q5KVY8Positive1286580 - 128705617639.2
hypothetical proteinAVP43_01304Not AvailableNegative1287079 - 12872405811.21
general stress protein 20uAVP43_01305P80879Positive1287391 - 128783116498.9
hypothetical proteinAVP43_01306P58702Positive1287985 - 128838014183.9
hypothetical proteinAVP43_01307Not AvailablePositive1288436 - 128877413470.6
putative 8-oxo-dgtp diphosphatase ytkdAVP43_01308O35013Positive1288835 - 128930217555.9
putative aliphatic sulfonates transport permease protein ssucAVP43_01309O34649Negative1289418 - 129022729923.4
bicarbonate transport atp-binding protein cmpcAVP43_01310O34314Negative1290211 - 129100229144.5

Displaying genes 1311 – 1320 of 6618 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolites

455 records
Metabolite IDMetabolite nameStructureCAS number
BASm0000173(R)-3-Hydroxybutyric acidC4H8O3Chemical structure of (R)-3-Hydroxybutyric acid625-72-3
Average104.0473Da
Monoisotopic104.047344122Da
BASm0000217alpha-ribazoleC14H18N2O4Chemical structure of alpha-ribazoleNot available
Average278.3037Da
Monoisotopic278.126657074Da
BASm0000237(R)-4'-phosphopantothenateC9H18NO8PChemical structure of (R)-4'-phosphopantothenateNot available
Average299.2149Da
Monoisotopic299.0770031Da
BASm00002512-dehydropantoateC6H9O4Chemical structure of 2-dehydropantoateNot available
Average145.1333Da
Monoisotopic145.050083776Da
BASm0000272(E)-4-coumarateC9H7O3Chemical structure of (E)-4-coumarateNot available
Average163.1501Da
Monoisotopic163.0395191Da
BASm0000274aldehydo-D-galacturonateC6H9O7Chemical structure of aldehydo-D-galacturonateNot available
Average193.132Da
Monoisotopic193.0353762Da
BASm0000377(S)-malateC4H4O5Chemical structure of (S)-malateNot available
Average132.0716Da
Monoisotopic132.005873238Da
BASm0000387(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6Chemical structure of (6R)-5,10-methylene-5,6,7,8-tetrahydrofolateNot available
Average455.432Da
Monoisotopic455.1564286Da
BASm0000399(S)-allantoinC4H6N4O3Chemical structure of (S)-allantoin97-59-6
Average158.1154Da
Monoisotopic158.0439901Da
BASm0000542HgHgChemical structure of HgNot available
Average200.59Da
Monoisotopic201.9706256Da

Displaying 1–10 of 455 metabolites

Health Effects

No health effects information available for this bacterium.